EFFECT OF BORATE ON A PROTEIN-POLYSACCHARIDE COMPLEX, THE PHOSPHOESTERASE FROM CALF INTESTINAL MUCOSA sirs: An enzyme preparation from calf intestinal mucosa
نویسنده
چکیده
An enzyme preparation from calf intestinal mucosa was studied for phosphodiesterase activity’ but was found to contain also phosphomonoesterase’ and adenosine deaminase.2 Analysis confirmed the presence of polysaccharide, suspected because of solubility in high concentrations of organic solvents and precipitation from aqueous solutions only when fully saturated with ammonium sulfate. Attempts were made to obtain a more active phosphodiesterase fraction with little success. It was concluded that enzymes and inert proteins had closely similar properties, perhaps all being protein-polysaccharide complexes.
منابع مشابه
Adenosine deaminase from calf intestinal mucosa.
In the course of studying the phosphoric acid esterases from calf intestinal mucosa (1, 2) the preparations were found to contain a potent deaminase for adenosine. The method of preparation is simple and the procedure indicates that this enzyme is quite stable. This deaminase is probably identical with that recently prepared by Brady (3) from the same material by a somewhat different procedure....
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